2016-02-10
Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3
Publication
Publication
Journal of Agricultural and Food Chemistry , Volume 64 - Issue 5 p. 1191- 1201
In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.
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doi.org/10.1021/acs.jafc.5b04401, hdl.handle.net/1765/89837 | |
Journal of Agricultural and Food Chemistry | |
Organisation | Erasmus MC: University Medical Center Rotterdam |
Reitsma, M., Bastiaan-Net, S., Sforza, S., van der Valk, H., Gerth van Wijk, R., Savelkoul, H., … Wichers, H. J. (2016). Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3. Journal of Agricultural and Food Chemistry, 64(5), 1191–1201. doi:10.1021/acs.jafc.5b04401 |